文献类型：会议

作　　者：曲丽辉 于磊 王艳丽 Xin Jin 张倩龙 陆萍 于秀峰 Ningren Cui Chun Jiang 朱大岭

作者单位：黑龙江省生物制药重点实验室 Department of Biology,Georgia State University,50 Decatur Street,Atlanta,GA 30302,USA 哈尔滨医科大学大庆校区药学院生物制药教研室 哈尔滨医科大学大庆校区基础医学院生理学教研室 哈尔滨医科大学第一临床医学院风湿免疫教研室

会议文献：中国生理学会第24届全国会员代表大会暨生理学学术大会论文汇编

会议名称：中国生理学会第24届全国会员代表大会暨生理学学术大会

会议日期：20141024

会议地点：中国上海

主办单位：中国生理学会

出版日期：20141024

学会名称：中国生理学会

语　　种：中文

摘　　要：Endothelium lines vascular walls and regulates vascular tones.The endothelial cells sense and respond to chemical and mechanical stimuli in the circulation,and couple the stimulus signals to vascular smooth muscles,in which several membrane receptors and ion channels play a role.Information about these surface-expressing proteins may help to understand endothelium function and its role in certain vascular diseases.Here we show a K+current in primarily cultured pulmonary arterial endothelial cells(PAECs)that is regulated by the Ca2+/calmodulin(Ca M)-dependent protein kinase Ⅱ(Ca MKⅡ).In whole-cell voltage clamp,the PAECs showed large inward rectifier K+currents that were sensitive to micromolar concentrations of extracellular Ba2+,but insensitive to intracellular ATP and p H.In excised inside-out patches,an inward rectifier K+current was observed with single-channel conductance 32.43±0.45 p S and Popen 0.27±0.04.Quantitative PCR analysis showed strong expression of Kir2.1 in the endothelial cells.The current did not respond to intracellular GDP-β-S and extracellular cholera toxin.Several vascular regulators were studied.This K+current was insensitive to carbachol,neither to the e NOS inhibitor L-NAME.Exposure to the Ca M inhibitor W-7 and Ca MKⅡ inhibitor KN-62 strongly suppressed the current.Consistently,vasodilation was suppressed by W-7 and Ba2+in isolated and perfused pulmonary arterial rings.Therefore,these results suggest that the PAECs express an inward rectifier K+current that is likely to be Kir2.1,and such a K+channel appears to be targeted by Ca MKⅡ-dependent intracellular signaling systems.

关 键 词：Inward rectifier K+ currents  endothelial cells  Ca MKⅡ  primarily cultured bovine pulmonary arteries  

分 类 号：R331[基础医学类]