文献类型：期刊文章

作　　者：范春玲[1] 李燕[1] 高平进[1] 刘建军[1] 张学军[2] 朱鼎良

机构地区：[1]上海第二医科大学附属瑞金医院上海市高血压研究所医学遗传学国家重点实验室,上海200025 [2]中国科学院上海生命科学研究院生物化学与细胞生物学研究所分子细胞生物学重点实验室

出　　处：《Acta Pharmacologica Sinica》

基　　金：Project supported by the grants from the National Key Program of Basic Research (№ G2000056904), the Shanghai Science and Technology Committee, and the Health Science Center,Shanghai Second Medical University and Chinese Academy of Sciences.

年　　份：2003

卷　　号：24

期　　号：3

起止页码：212-218

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、FSTA、IC、JST、PROQUEST、PUBMED、SCI-EXPANDED(收录号：WOS:000181439200004)、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：AIM: To study the time course of the expression of stem cell marker and endothelial cell markers on human cord blood CD34^+ cells during in vitro differentiation process of endothelial progenitor cells (EPC). METHODS: CD34^+ cells were selected and enriched from human cord blood by magnetically activated cell sorting (MACS), and cultured in dishes coated with or without fibronectin (Fn). Endothelial cells were identified by staining the cells with anti Flk-1 and vWF antibodies. The percentage of AC133^+ cells in adherent CD34^+ cell population was analyzed by fluorescence-activated cell sorting (FACS). RESULTS: The expression of Flk-1 and vWF on adherent CD34^+ cells increased during the culture time, with 27.0％positive for Flk-1 and negative for vWF at d 3, and 100％positive for both Flk-1 and vWF at d 7. When cells were cultured in Fn-treated dishes, the percentages of Flk-1 and vWF positive cells increased to 34％and 47％, respectively at d 3, and 100％at d 7. In contrast, the percentages of AC 133^+ cells among the adherent cell population decreased rapidly, and similar changes occurred in cells cultured in the presence of Fn. CONCLUSION: The gradual appearance of endothelial cell markers and the disappearance of stem cell marker characterized the in vitro differentiation of endothelial progenitor cells. Fibronectin accelerated the differentiation process of EPC.

关 键 词：vascular endothelium  cell differentiation  CD34^+ antigens  pregency  genetic markers  hematopoietic stem cells  FIBRONECTINS

分 类 号：R329.2]