文献类型：期刊文章

作　　者：骆静[1] 尹江华[1] 吴和珍[1] 魏群[1]

机构地区：[1]北京师范大学生命科学学院生物化学及分子生物学系

出　　处：《Acta Pharmacologica Sinica》

基　　金：Project supported by the National Natural Science Foundation of China (№ 39930060) and the State Key Basic Research and Development Program (№ 1998051123).

年　　份：2003

卷　　号：24

期　　号：11

起止页码：1137-1142

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、FSTA、IC、JST、PROQUEST、PUBMED、SCI-EXPANDED(收录号：WOS:000186505500014)、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：AIM: To investigate the effects of extract from Fructus cannabis(EFC) that can activate calcineurin on learning and memory impairment induced by chemical drugs in mice. METHODS: Bovine brain calcineurin and calmodulin were isolated from frozen tissues. The activity of calcineurin was assayed using p-nitrophenyl phosphate(PNPP) as the substrate. Step-down type passive avoidance test and water maze were used together to determine the effects of EFC on learning and memory dysfunction. RESULTS: EFC activated calcineurin activity at a concentration range of 0.01-100g/L. The maximal Value of EFC on calcineurin activity(35%±5%) appeared at a concentration of 10g/L. The chemical drugs such as scopolamine, sodium nitrite, and 45% ethanol, and sodium pentobarbital induced learning and memory dysfunction. EFC administration(0.2, 0.4, and 0.8g/kg, ig×7d) prolonged the latency and decreased the number of errors in the step-down test. EFC, given for 7d, enhanced the spatial resolution of amnesic mice in water maze test. EFC overcome amnesia of three stages of memory process at the dose of 0.2g/kg. CONCLUSION: EFC with an activation role of calcineurin can improve the impaired learning and memory induced by chemical drugs in mice.

关 键 词：Fructus cannabis  CALCINEURIN avoidance learning  maze learning  memory disorders  

分 类 号：R285[中药学类]