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期刊文章详细信息

猪圆环病毒2型及猪繁殖与呼吸综合症病毒的快速检测    

Rapid Detection of Porcine circovirus Type 2 and Porcine reproductive and respiratory syndrome virus

  

文献类型:期刊文章

作  者:芦银华[1] 许立华[1,2] 华修国[3] 谈国蕾[1] 黄伟坚[1] 陈德胜[1] 陈溥言[1]

机构地区:[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室 [2]宁夏大学农学院动物科学系,宁夏银川750105 [3]上海交通大学农学院

出  处:《中国病毒学》

基  金:国家863高技术发展计划资助项目(2001AA249012);上海市科技兴农攻关项目[农科攻字(2001)第3-5号]

年  份:2003

卷  号:18

期  号:2

起止页码:184-186

语  种:中文

收录情况:BIOSISPREVIEWS、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EMBASE、IC、PUBMED、ZGKJHX、普通刊

摘  要:According to the published genome sequences of Porcine circovirus type 2 (PCV2) and Porcine reproductive and respiratory syndrome virus (PRRSV), primers were designed and PCR, RT-PCR were set up for the detection of PCV2 and PRRSV, respectively. With the established methods, 38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 and PRRSV. The results demonstrated that 22 samples were positive for PCV2, 27 samples were positive for PRRSV and among the above positive samples, 18 samples were positive for both viruses. The data obtained in the present study indicated that PCV2 and PRRSV maybe play an important role in the course of the development of respiratory diseases.

关 键 词:猪圆环病毒2型 猪繁殖呼吸综合症病毒  共感染 检测  呼吸道疾病 PCR RT-PCR

分 类 号:S852.65]

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