文献类型：期刊文章

作　　者：张世英[1] 洪帮兴[1] 司徒潮满[1] 钟跃星[1] 田华伟[1] 黄慈林[1] 张雪[1]

机构地区：[1]深圳市福田区疾病预防控制中心,518033

出　　处：《中国公共卫生》

基　　金：深圳市暨福田区科技三项经费资助

年　　份：2003

卷　　号：19

期　　号：3

起止页码：345-346

语　　种：中文

收录情况：BDHX、BDHX2000、CAS、CSCD、CSCD_E2011_2012、JST、RCCSE、ZGKJHX、核心刊

摘　　要：Objective To construct the detection method of vibrio cholerae with fluorescence quantitative PCR and evaluate its superiority.Methods A diagnostic kit(FQ-PCR) was developed according to the common sequence of NhaA gene in vibrio cholerae.The sensitivity and speciality in detecting of vibrio cholerae in infectious water,sea food and winter-endurance specimen and clinical samples were compared with FQ-PCR kit.Results In the detection of clinical samples,FQ-PCR and regular detection ways have the same sensitivity and speciality.While in the detection of infectious water,sea food and winter-endurance specimen,FQ-PCR has superiority to regular ways.Conclusion The construction of FQ-PCR method eliminates PCR cross-contamination which cause false positive,and real-time detection ensure accurate quantity.So FQ-PCR can be used to the detection of vibrio cholerae in clinical and sanitary laboratory.

关 键 词：霍乱弧菌 聚合酶链反应 荧光光度测定法 应用  

分 类 号：R378.3]