文献类型：期刊文章

作　　者：秦庆明[1] 张全[1] 赵文生[1] 王云月[2] 彭友良[1]

机构地区：[1]中国农业大学植物病理系,北京100094 [2]云南农业大学植物病理系,昆明650201

出　　处：《Acta Botanica Sinica》

基　　金：国家重点基础研究发展规划项目(G2000016204);云南省中国农业大学省校合作项目(98N08)~~

年　　份：2003

卷　　号：45

期　　号：1

起止页码：76-81

语　　种：中文

收录情况：CSCD、CSCD2011_2012、SCI-EXPANDED(收录号：WOS:000180476600011)、SCOPUS、WOS、普通刊

摘　　要：By mRNA differential display, eight induced cDNAs were obtained from rice leaves infected with an incompatible race 131 of Magnaporthe grisea, and one of these cDNAs was highly similar to salt-induced mannose-binding lectin gene. Using this fragment as a probe, a full length cDNA was isolated from a nice cDNA library, which was constructed using mRNA from the incompatible race-infected leaves. Sequence analysis indicates that the cDNA encodes a protein of 15 kD with 145 amino, acids and shares 96% identity at nucleotide level with MRL and salT, but is identical to MRL at amino acid level. Genomic Southern blotting shows that there are two mannose-binding lectin genes in rice genome. Northern blotting analysis indicates that the gene was strongly and specifically induced in rice leaves infected with the incompatible race, suggesting that the lectin induction be involved in the defense of rice to M. grisea.

关 键 词：RICE mRNA differential display  LECTIN Magnaporthe grisea  

分 类 号：S435.111]