文献类型：期刊文章

作　　者：何晓晓[1] 王柯敏[1] 谭蔚泓[1] 刘斌[1] 李杜[1] 黄杉生[1]

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室,湖南大学化学化工学院,湖南大学生物技术研究院,长沙410082

出　　处：《高等学校化学学报》

基　　金：科技部基础研究重大项目前期研究专项基金 (批准号 :2 0 0 1-5 1);国家自然科学基金重点项目 (批准号 :2 0 135 0 10 );教育部重大项目基金 (批准号 :2 0 0 0-15 6 )资助

年　　份：2003

卷　　号：24

期　　号：1

起止页码：40-42

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、CAS、CSCD、CSCD2011_2012、EI、IC、JST、RCCSE、RSC、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：The present paper covers a novel technology for the concentration of trace amounts of target oligonucleotide from the solution. This technique is based on a super-paramagnetic DNA nano-enricher constructed with a single strand DNA probe immobilized onto the surface of the super-paramagnetic nanoparticles prepared by using the water-in-oil microemulsion technique, employing silica as the shell and iron oxide as the core of the super-paramagnetic nanoparticles. The silica coated magnetic nanoparticles are (40±4) nm in size. And the magnetic nanoparticle is super-paramagnetic. Biotin labeled ssDNA(Biotin-5-(A)10-GAT-TCA-CGA-GGC-CCT-AGT-CG-3) was immobilized on the surface of silica coated magnetic nanoparticles. The complementary ssDNA could be enriched effectively and the characteristics of the enriched ssDNA have not changed, which will provide a novel technique and measurement for gene transfection, mutation detection, gene diagnosis, gene therapy and so on.

关 键 词：超顺磁性 DNA 纳米富集器  富集  纳米颗粒  分离  四氧化三铁 二氧化硅  

分 类 号：Q523]