文献类型：期刊文章

作　　者：廖晓玲[1] 郎惠云[2] 汪海涛[2] 李亚荣[2]

机构地区：[1]汉中农业学校,陕西汉中723000 [2]西北大学化学系,陕西西安710069

出　　处：《药学学报》

年　　份：2002

卷　　号：37

期　　号：12

起止页码：996-998

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAB、CAS、CSCD、CSCD2011_2012、DOAJ、EMBASE、IC、IPA、JST、PUBMED、RCCSE、RSC、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：AIM To establish a flow injection chemiluminescence (CL) inhibition method for determination of tea polyphenols in green tea. METHODS The flow line of the sample, H 2O 2 and KIO 4 was inserted into luminol carrier solution. The mixed solution was led into the chemiluminescence cell for chemiluminescence reaction to occur and the CL intensity was recorded. RESULTS Tea polyphenols was shown to inhibit the CL reaction of luminol-H 2O 2-KIO 4 system in alkaline solution and the CL emission was inversely correlated with the tea polyphenols concentration. The linear range was 0.4～24.0 μg·mL -1 with a detection limit of 0.03 μg·mL -1 and a relative standard deviation of 1.3% (at tea polyphenols concentration 4.0 μg·mL -1, n=11). The sampling frequency was 240·h -1. CONCLUSION The method is simple, rapid and can be used for determination of tea polyphenols in green tea.

关 键 词：绿茶 茶多酚 流动注射 化学发光 含量测定  

分 类 号：R917] R927.2[药学类]