文献类型：期刊文章

作　　者：周航[1,2] 李凤[1,2] 牛建一[1,2] 钟伟勇[1,2] 汤敏誉[1,2] 林栋[1,2] 崔洪珲[1,2] 黄学涵[1,2] 陈莹莹[1,3] 王红艳[4] 涂永生[1]

ZHOU Hang;LI Feng;NIU Jian-Yi;ZHONG Wei-Yong;TANG Min-Yu;LIN Dong;CUI Hong-Hui;HUANG Xue-Han;CHEN Ying-Ying;WANG Hong-Yan;TU Yong-Sheng(Department of Physiology,School of Basic Medical Sciences,Guangzhou Medical University,Guangzhou 511436,China;The First Clinical Medical College,Guangzhou Medical University,Guangzhou 511436,China;The Sixth Clinical Medical College,Guangzhou Medical University,Guangzhou 511436,China;Department of Pathology,School of Basic Medical Sciences,Guangzhou Medical University,Guangzhou 511436,China)

机构地区：[1]广州医科大学基础医学院生理教研室,广州511436 [2]广州医科大学第一临床学院,广州511436 [3]广州医科大学第六临床学院,广州511436 [4]广州医科大学基础医学院病理教研室,广州511436

出　　处：《生理学报》

基　　金：supported by the Natural Science Foundation of Guangdong Province,China(No.2018A0303130256);the Medical Scientific Research Foundation of Guangdong Province,China(No.A2017315,A2018390 and A2019042);the Laboratory Open Project for College Students of Guangzhou Medical University;the National College Students’ Innovation Entrepreneurship Training Program of China(No.20181057000);the Guangzhou Medical University College Students Science Technology Innovation Project(No.2017A006);Special Funds for the Cultivation of Guangdong Provincial College Students’ Scientific and Technological Innovation; “Climbing Program” Special Funds(No.pdjhb0419)

年　　份：2019

卷　　号：71

期　　号：5

起止页码：689-697

语　　种：中文

收录情况：BDHX、BDHX2017、BIOSISPREVIEWS、CAS、CSCD、CSCD2019_2020、EMBASE、IC、JST、PUBMED、RCCSE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：本研究旨在探讨铁死亡在油酸(oleic acid, OA)致急性肺损伤(acute lung injury, ALI)小鼠模型中的作用。小鼠尾静脉注射纯OA制备ALI模型,以肺组织病理学评分、肺湿干重比、支气管肺泡灌洗液(bronchoalveolar lavage fluid, BALF)蛋白含量作为ALI的评价指标,用试剂盒检测肺组织的铁浓度、谷胱甘肽(glutathione, GSH)和丙二醛(malondialdehyde, MDA)含量,用透射电子显微镜观察肺细胞的超微结构,用定量PCR (q-PCR)检测肺组织中前列腺素内过氧化物合酶2 (prostaglandin-endoperoxide synthase 2, PTGS2) mRNA表达,用Western blot测定肺组织谷胱甘肽过氧化物酶4 (glutathione peroxidase 4, GPX4)、铁蛋白和转铁蛋白受体1 (transferrin receptor 1, TfR1)的蛋白表达水平。结果表明,OA组小鼠肺组织病理评分、肺湿干重比和BALF中蛋白均高于对照组。OA组小鼠肺细胞线粒体缩小,线粒体膜破裂。与对照组相比,OA组PTGS2 mRNA表达增加7倍。OA组小鼠肺组织出现铁过载、GSH含量减少和MDA累积。与对照组相比,OA组小鼠肺组织GPX4和铁蛋白表达水平下调。以上结果提示,铁死亡可能在ALI发病机制中发挥作用,这为治疗ALI的新药开发提供了新的角度。

关 键 词：铁死亡  油酸 急性肺损伤 谷胱甘肽 谷胱甘肽过氧化物酶4  铁

分 类 号：R363]