文献类型：期刊文章

作　　者：庄辉[1] 毕胜利[2] 王佑春[3] 李凡[1] 朱万孚[1] 朱永红[1] 李奎[1]

机构地区：[1]北京大学基础医学院病原生物学系,北京100083 [2]中国疾病控制预防中心病毒病控制预防所 [3]中国药品生物制品检定所

出　　处：《北京大学学报（医学版）》

基　　金：国家"七五";"八五"和"九五"医学科技攻关项目 ( 75 62 0 1 0 9;85 91 6 0 1 0 4 ;96 90 6 0 3 0 7)

年　　份：2002

卷　　号：34

期　　号：5

起止页码：434-439

语　　种：中文

收录情况：BDHX、BDHX2000、CAS、CSCD、CSCD2011_2012、IC、JST、PUBMED、SCOPUS、ZGKJHX、核心刊

摘　　要：An epidemic of hepatitis in the south part of Xinjiang Uighur Autonomous Reg ion during 1986-1988, and 2 548 acute sporadic hepatitis cases from 17 cities of Ch i na were studied. The disease had following clinical and epidemiological char acte ristics: 86.4% cases occurred in the age group of 20-59 years; with male prepon d erance and autumn-winter excess; 75% patients had a history of contact with hep a titis cases, and / or eating out or drinking unboiled water; clinical features w ere s imilar to hepatitis A, with a higher fatality rate, especially in pregnant women (up to 21%). Two strains of hepatitis E virus (HEV) isolated from Xinjiang were completely seq uenced by dideoxy chain termina tion method, with nucleotide homology of 93.5% and 94.5% with Burma strain (geno type 1), 75.7% and 75.9% with Mexico strain (genotype 2), 73.7% and 74.2 % with American strain (genotype 3), respectively. It suggests that these two st rains belong to genotype 1. The partial sequencing of open reading frame (ORF)2 region of 98 HEV isol ates from 19 cities of China revealed that 62 of them (63.3%) shared the same ge notype with Burma and Xinjiang strains, and 36 (36.7%) isolates were of genotype 4. One of the 36 strains was completely sequenced, with nucleotide identity of 75% with Burma strain, 74.5% with Mexico strain and 75.3% with American strain , respectively. Sera collected from 419 pigs, 190 cattle and 316 goats from var ious regions of China were detected for anti-HEV antibodies and HEV RNA using a n in-house enzyme immunoassay (EIA) and reverse transcriptase nested polymerase chain re action (RT-nPCR) with primers from ORF 2. The mean positivity rates of anti-HEV antibody for pigs and cattle were 78.8% and 6. 3%, respectively, but none of the goat sera was anti-HEV positive. The PCR product s (nt 6007- 6354) of 5 HEV RNA positive sera were sequenced and compared to othe r HEV sequences in the nucleotide databases. The five sequences shared 74%-79%, 73%-74%, 73%-78%, and 83%-99% identity to HEV genotypes 1,2,3 and 4

关 键 词：戊型肝炎 戊型肝炎病毒 分离  提纯 新疆维吾尔自治区  流行病学

分 类 号：R512.63]