文献类型：期刊文章

作　　者：何韶衡[1] 陈谱[2] 陈韩秋[3]

机构地区：[1]汕头大学医学院变态反应学与炎症学研究所 [2]汕头大学医学院变态反应学与炎症学研究所 汕头 515031 中国 [3]汕头 515031 中国

出　　处：《Acta Pharmacologica Sinica》

基　　金：Project supported by the National Natural Science Foundation of China, № 30140023.

年　　份：2003

卷　　号：24

期　　号：9

起止页码：85-91

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、FSTA、IC、JST、PROQUEST、PUBMED、SCI-EXPANDED(收录号：WOS:000185357500015)、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：AIM: To investigate the actions of protease inhibitors on the enzymatic activities of tryptase and chymase in similar experimental systems. METHODS: Human lung tryptase and human skin chymase were purified by a similar procedure involving high salt extraction of tryptase, heparin agarose affinity chromatography, and S-200 Sephacryl gel filtration chromatography. Actions of protease inhibitors on tryptase and chymase activities were examined by enzyme assays. RESULTS: The specific activities of tryptase and chymase were 2.1 kU/g protein and 4.9 kU/g protein, respectively. Both preparations showed a single diffuse band on SDS-PAGE. Among non-native protease inhibitors, N-（1-hydroxy-2-naphthoyl）-L-arginyl-L-prolinamide hydrochloride （HNAP）, leupeptin, antipain, benzamidine, and protamine inhibited more than 90% enzymatic activity of tryptase, whereas soy bean trypsin inhibitor （SBTI）, Z-Ile-Glu-Pro-Phe-CO₂Me （ZIGPPM） and chymostatin inhibited more than 95% enzymatic activity of chymase. Native protease inhibitos α₁-antitrypsin and secretory leukocyte protease inhibitor （SLPI） inhibited more than 90% enzymatic activity of chymase, but lactoferrin appeared to enhance chymase enzymatic activity. All the 3 inhibitors had weak inhibitory actions on tryptase. CONCLUSION: The protease inhibitors tested had relatively good selectivity to either tryptase or chymase.

关 键 词：TRYPTASE CHYMASE mast cells  LACTOFERRIN protease inhibitors  

分 类 号：R96]