文献类型：期刊文章

作　　者：禹永春[1] 郭慧淑[1] 朴琳[1] 李林[1] 李在琉[1] 许文燮

机构地区：[1]延边大学医学院消化生理研究室,延吉中国133000

出　　处：《Acta Pharmacologica Sinica》

基　　金：Project supported by the National Natural Science Foundation of China, No 3980031.

年　　份：2002

卷　　号：23

期　　号：11

起止页码：49-54

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、FSTA、IC、JST、PROQUEST、PUBMED、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：AIM: To investigate the role of intracellulaf calcium in muscarinic currents increased by hypoosmotic membrane stretch in gastric antral circular myocytes of the guinea pig. METHODS: The whole cell patch-clamp technique was used, and the myocytes were isolated by collagenase. Cells were swelled by hypoosmotic solution (200 Osmmol/kg). RESULTS: The hypoosmotic membrane stretch markedly increased carbachol-induced muscarinic currents (Icch ). The Icch and the increase of Icch were completely blocked by quinidine 3 μmol/L, a specific musca-rinic current blocker. In external calcium-free solution hypoosmotic membrane stretch could not increase Icch , but in the presence of nicadipine 5 μmol/L, a L-type calcium channel blocker or gadolinium chloride 100 nmol/L, a stretch-activated cation channel blocker, the Icch was still increased by hypoosmotic membrane stretch. When both nicardipine and gadolinium chloride were added into external solution, Icch were not increased by hypoosmotic membrane stretch any more. Ryanodine, a calcium-induced calcium release (CICR) agonist completely blocked hypoosmotic membrane stretch-induced increase of Icch . CONCLUSION: Hypoosmotic membrane stretch in-creased Icch and the increment was related to influx of external calcium and CICR

关 键 词：muscarinic receptors  pyloric antrum  membrane stretch  hypotonic solusions  calcium  CARBACHOL patch-clamp techniques  

分 类 号：R333]