文献类型：期刊文章

作　　者：刘正春[1,2] 贺全国[1] 肖鹏峰[1] 梁波[3] 何农跃[1] 陆祖宏[1]

机构地区：[1]东南大学国家教育部分子与生物分子电子学开放实验室 [2]怀化师范高等专科学校化学系,怀化418008 [3]湘潭大学化学学院

出　　处：《高等学校化学学报》

基　　金：国家八六三计划 (批准号 :10 3 -13 -0 5 -0 110 1-0 1-0 1);九七三计划 (批准号 :G19980 5 12 0 4);国家自然科学基金 (批准号 :60 0 710 0 1);教育部"高等学校骨干教师资助计划"项目资助

年　　份：2002

卷　　号：23

期　　号：9

起止页码：1823-1825

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、CAS、CSCD、CSCD2011_2012、EI、IC、JST、RCCSE、RSC、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：Polyurethane based on polypropylene glycol(PPG) and TDI using 3,3\|dichloride\|4,4\|methylenedianiline(MOCA) as the crosslinker is presented for the first time to fabricate molecular stamps(PU stamps) for the synthesis of DNA microarray with contact procedure. The predictability of the process is achieved by utilizing commercially available starting materials. SEM analysis of the morphology of PU stamps and photoresist motherboard showed that PU elastomer could replicate subtly the motherboards patterns with a high fidelity. It was proved from the contact angle measurement that PU stamps surface has a good affinity with acetonitrile, which guarantee the well\|distribution of DNA monomers on patterned stamps. Laser confocal fluorescence microscopy images of oligonucleotide arrays confirm that polyurethane is an excellent material for molecular stamps.

关 键 词：制备  表征  分子印章  聚氨酯 软平板印刷  DNA微阵列 NA芯片  

分 类 号：Q523] O633.14]