文献类型：期刊文章

作　　者：傅洪哲[1,2] 宋佳芳[1,2] 宋歌[1,2] 宋思洋[1,2] 樊志璞[1,2] 杨岸蒲[1,2] 何冰[1,2] 代文兵[1,2] 张华[1,2] 王学清[1,2] 张强[1,2]

机构地区：[1]北京大学医学部药学院天然药物及仿生药物国家重点实验室 [2]药学院药剂学系,北京100191

出　　处：《Journal of Chinese Pharmaceutical Sciences》

基　　金：National Basic Research Program of China(973Pro gram,Grant No.2015CB932100);National Natural Science Foundation of China(Grant No.81130059)

年　　份：2017

卷　　号：26

期　　号：6

起止页码：395-403

语　　种：中文

收录情况：CAB、CAS、CSCD、CSCD2017_2018、DOAJ、EMBASE、IC、JST、SCOPUS、ZGKJHX、普通刊

摘　　要：Rho GTPases play an important role on the regulation of cytoskeleton, which can affect the cell morphogenesis, cell migration, endocytosis and vesicle transport by controlling the growth and maintenance of microfilaments and microtubules. It has been known that regulation of cell cytoskeleton is inseparable from the cell uptake of nano-medicine or nano-drug delivery systems. However, only few studies have focused on the impacts of Rho GTPases on cell uptake of nano-medicine or nano-drug delivery systems. This study selected single-walled carbon nanohoms （SWCNHs）, which have emerged as promising drug delivery systems, to explore the impacts of Rho GTPases on cell uptake of nano-drug delivery systems. SWCNHs were oxidized with concentrated nitric acid and prepared into nano dispersion by ultrasonic dispersion. Confocal laser scanning microscope （CLSM） and transmission electron microscopy （TEM） were used to observe the cell uptake and intracellular distribution of nanoparticles after incubated A549 cells with the dispersion mentioned above. Mechanism of cell uptake was assessed using various inhibitors. The results showed that the cell uptake of oxSWCNHs was significantly reduced when RhoA was inhibited. The oxSWCNHs were internalized through clathrin-mediated endocytosis and mainly positioned in lysosomes ofA549 cells.

关 键 词：Rho GTPases  Single-walled carbon nanohoms  A549 cells  Cell uptake  

分 类 号：R943]