文献类型：期刊文章

作　　者：王蕾[1] 巴春影[1] 曹后男[1] 宗成文[1] 姚航[1]

机构地区：[1]延边大学农学院园艺园林系,吉林延边133002

出　　处：《Agricultural Science & Technology》

基　　金：Supported by Students'Innovation and Entrepreneurship Training Program of Yanbian University in 2015(ydbksky2015252)~~

年　　份：2015

卷　　号：16

期　　号：7

起止页码：1348-1351

语　　种：中文

收录情况：CAB、CAS、CSA、CSA-PROQEUST、PROQUEST、普通刊

摘　　要：[Objective] This study aimed to shorten the multiplication culture and root- ing culture periods of Rh. chrysanthum Pall. [Method] The Rh. chrysanthum Pall tis- sue culture plantlets collected from Changbai Mountain were used as material, and the effects of different hormone combinations and coconut milk on the proliferation and differentiation of Rh. chrysanthum Pall tissue culture plantlets were investigated. In addition, the rooting medium and transplanting matrix for Rh. chrysanthum Pall tissue culture plantlets were explored. [Result] The medium composed of modified MS, iBA （3 mg/L） and ZT （1.5 mg/L） was the optimum medium for subculture mul- tiplication of Rh. chrysanthum Pall tissue culture plantlets. The multiplication multiple and average plant height were significantly improved by adding coconut milk into the medium （150 mg/L）. [Conclusion] For Rh. chrysanthum Pall tissue culture plantlets, the optimum rooting culture medium was composed of modified MS （1/4） and IBA （5.0 mg/L）, and the tissue culture plantlets began to root 8 d after the inoculation. The root induction treatment was carried out after a 15-d sand culture, and the suitable matrix was composed of tufty soil, humus soil and perlite （2：1：1） with a survival rate of 95.66%.

关 键 词：Rh. chrysanthum Pall  Multiplication culture  ROOTING Transplanting matrix  

分 类 号：S685.21]