文献类型：期刊文章

作　　者：赵欣[1,2] 张世平[1,2] 安春娜[1,2] 张宏宁[3] 孙懿[1,2] 李艳梅[3] 蒲小平[1,2]

机构地区：[1]北京大学医学部药学院分子与细胞药理学系 [2]天然药物及仿生药物国家重点实验室,北京100191 [3]北京绿色金可生物技术股份有限公司,北京100044

出　　处：《Journal of Chinese Pharmaceutical Sciences》

基　　金：National Natural Science Foundation of China(Grant No.81202937);the National Key Scientific Instrument and Equipment Development Project(Grant No.2013YQ030651);the New Teachers’ Fund from School of Pharmaceutical Sciences,Peking University(Grant No.BMU20100090)

年　　份：2015

卷　　号：24

期　　号：7

起止页码：467-474

语　　种：中文

收录情况：CAB、CAS、CSCD、CSCD2015_2016、DOAJ、EMBASE、IC、JST、SCOPUS、ZGKJHX、普通刊

摘　　要：Alzheimer＇s disease （AD） is one of the most common cognitive disorders of the elderly. Fucoxanthin is a carotenoid that is found in common edible seaweed, and it is considered as a major active compound of marine algae with cancer-preventing, antioxidant and anti-inflammatory properties. In this study, we investigated the ability of fucoxanthin to protect against the β-amyloid protein （Aβ）-induced neurotoxicity in primary cortical cultured neurons and PC12 cells. Neuroprotective effects of fucoxanthin were determined by measuring cell viability and nuclei double-staining with Hoechst 33342 and propidium iodide following Aβ treatment with or without fucoxanthin. Moreover, we also evaluated its potential mechanism on antioxidation by detecting the total antioxidant capacity （T-AOC）, level of lipid peroxidation malondialdehyde （MDA） and activity of superoxide dismutase （SOD）. We found that exposure of cortical cultured neurons or PC12 cells to Aβ resulted in neuronal cell death, whereas pre-treatment with fucoxanthin reduced Aβ-induced cell death. The data on the T-AOC, MDA level and SOD activity showed that Aβ treatment resulted in decreases in T-AOC and SOD activity and an increase in MDA level. After fucoxanthin administration, the results of T-AOC, MDA level and SOD activity showed an opposite trend, indicating that T-AOC was increased and MDA level was reduced. These results suggested that fucoxanthin prevented Aβ-induced neurotoxicity through attenuating oxidative stress induced by Aβ. Therefore, fucoxanthin might be useful as a potential preventive or theraoeutic agent for AD.

关 键 词：Alzheimer＇s disease  Neurodegenerative disorder  ANTIOXIDATION

分 类 号：R285[中药学类]