文献类型：期刊文章

作　　者：谢雁景[1] 刘子光[1] 高雅宁[1] 王秀伶[1] 郝庆红[1] 于秀梅[1]

机构地区：[1]河北农业大学生命科学学院制药工程系,河北保定071000

出　　处：《Journal of Chinese Pharmaceutical Sciences》

基　　金：National Natural Science Foundation of China(Grant No.31170058);the Service Center for Experts and Scholars of Hebei Province(Grant No.CPRC027)

年　　份：2015

卷　　号：24

期　　号：7

起止页码：442-448

语　　种：中文

收录情况：CAB、CAS、CSCD、CSCD2015_2016、DOAJ、EMBASE、IC、JST、SCOPUS、ZGKJHX、普通刊

摘　　要：A newly isolated bacterium, named as AUH-JLC257, was found to be capable of bioconverting isoflavone genistein to 5-hydroxy-equol under anaerobic conditions. The metabolite 5-hydroxy-equol was identified by using UV spectrum, electrospray ionization mass spectrometry （ESI-MS） as well as IH and 13C NMR analyses. Chiral stationary-phase high-performance liquid chromatography analysis and specific rotation examination demonstrated that the bio-synthesized 5-hydroxy-equol was just （-）-5-hydroxy-equol. The average bioconverting rate was 83.1%, and the strain AUH-JLC257 could efficiently transform genistein at a maximal substrate concentration of 0.6 mmol/L. We, for the first time, showed that the bio-synthesized 5-hydroxy-equol had 1,1-diphenyl-2-picrylhydrazyl （DPPH） radical-scavenging activity at concentrations as low as 3.3μmol/L. In addition, the 16S rRNA gene sequence （1401 bp） of the bacterium strain AUH-JLC257 showed the highest similarity （99.27%） to that of Slackia equolifaciens strain DZE.

关 键 词：GENISTEIN 5-Hydroxy-equol  Bacterial isolation  Identification  Microbial biotransformation  

分 类 号：Q93]