文献类型：期刊文章

作　　者：孔德强[1] 高慧媛[1] 李辛[2] 路金才[1] 袁丹[1]

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]沈阳药科大学医疗器械学院,辽宁沈阳110016

出　　处：《Journal of Chinese Pharmaceutical Sciences》

基　　金：The National Science and Technology Support Program during the Twelfth Five-Year Plan of China(Grant No.2011BAI03B05);the Distinguished Professor Foundation of Liaoning Province of China of 2011;Innovative Drug Incubation Base Plan Project from Liaoning Province of China of 2013(Grant No.2013226027)

年　　份：2015

卷　　号：24

期　　号：6

起止页码：364-375

语　　种：中文

收录情况：CAB、CAS、CSCD、CSCD2015_2016、DOAJ、EMBASE、IC、JST、SCOPUS、ZGKJHX、普通刊

摘　　要：Aristolochic acids （AAs） and aristololactams （ALs） are commonly found in some Aristolochiaceae plants, and they have been reported to be AA nephropathy （AAN）, nephrotoxicity and carcinogenicity. In the present study, we established an ultra high performance liquid chromatography （UHPLC） coupled with quadmpole/time-of-flight mass spectrometry （Q/TOF-MS） method for the rapid analysis of eight AA analogues in 19 samples originated from the five Aristolochiaceae plants, the roots and rhizomes ofAsarum sieboldii Miq. var. seoulense Nakai, the fruits ofAristolochia contorta Bunge or A. debilis Sieb. et Zucc., the roots ofAristolochia debilis Sieb. et Zucc., the stems ofAristolochia manshuriensis Kom., and the roots of Aristolochiafangchi Y. C. Wu ex L. D. Chou et S. M. Hwang. A total of five AAs and three ALs were identified by co-chromatography of sample extract and comparing the retention time, UV spectra, and characteristic molecular ions and fragment ions with those of authentic standards, or tentatively identified by MS/MS determination along with Mass Fragment software. Moreover, the method was validated for the simultaneous quantification or semi-quantification of them. The samples significantly differed in the quality and quantity of AA analogues, which aUowed the possibility of showing their chemical distinctness, and it might be helpful in their standardization and quality control. Furthermore, in order to holistically compare the difference between the five Aristolochiaceae plants, dataset obtained from UHPLC-Q/TOF-MS was processed with principal component analysis （PCA） and orthogonal partial least squared discriminant analysis （OPLS-DA）.

关 键 词：Aristolochic acids  Aristololactams  ARISTOLOCHIACEAE UHPLC-Q/TOF-MS  Quantification  Multivariate statistical analysis  

分 类 号：R284[中药学类]