文献类型：期刊文章

作　　者：黄坚[1] 陈苏红[1] 佟丽[1] 管伟[1] 丁雨[2] 梁好[2] 李五举[1] 王升启[1]

机构地区：[1]军事医学科学院放射医学研究所全军生物芯片重点实验室,北京100085 [2]深圳益生堂生物企业有限公司,深圳518026

出　　处：《生物工程学报》

基　　金：全军医药卫生科研基金课题 (No.0 1Z0 19);北京市科技项目 (No .H0 10 2 10 2 2 0 113)资助~~

年　　份：2002

卷　　号：18

期　　号：4

起止页码：501-504

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、IC、JST、PUBMED、RCCSE、SCOPUS、ZGKJHX、核心刊

摘　　要：A new method for the preparation of oligonucleotide microarray for gene expression detection was found. The key techniques and standards of quality controlling for preparation of oligonucleotide microarray was explored using gene of human 23kD highly protein and Luciferase and mouse cytokine-associated genes. By the using of a software system MProbe, oligonucleotide probes were designed and BLAST. All the probes have a very high specificity, i.e. except target sequence, the similarity between the probe and non-target sequences is less than 70% and the hairpin structure are not exist in all probes.All the probes have the same length 40. GC contents in all probes are in a narrow scope (from 45% to 55%). All the probes are modified with amino at 5′ or 3′ terminal. The satisfied images with good sensativity and very high specificity were obtained by the using of the methods above and also using of positive and negative controls and some internal controls(house keeping gene) to quantitate and balance expression of genes. High specificity, good sensativity and stablity have been verified by three continuous experiments using the oligonucleotide microarray to study gene expression profile of normal mouse breast grand tissue .The oligonucleotide microarray for expression detection prepared using our method have high specificity, good sensativity and stablity et al . It may be a more advanced method for analysis of gene expression profile.

关 键 词：小鼠 细胞因子 相关基因表达检测  寡核苷酸芯片 制备  基因表达谱

分 类 号：Q789]