文献类型：期刊文章

作　　者：姚伦广[1] 杜昌升[1] 彭建新[1] 洪华珠[1]

机构地区：[1]华中师范大学昆虫学研究所,武汉430079

出　　处：《微生物学报》

基　　金：国家留学基金资助项目

年　　份：2002

卷　　号：42

期　　号：3

起止页码：380-383

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAS、CSA-PROQEUST、CSCD、CSCD2011_2012、IC、JST、PUBMED、SCOPUS、ZGKJHX、核心刊

摘　　要：Microscopic examination of Spodoptera litura(SL-1)cell infected with AcMNPV or SfaMNPV revealed progressive cell blebbing starting at 8h～12h postinfection and culminating in total cell destruction at 24h postinfection.The fragmentation of the infected cell nuclei was observed by stained with the specific fluorescent dye DAPI.Agarose gel electrophoresis analysis of the DNA extracted from infected cells show typical DNA ladder. All these supported that both viruses infected SL-1 cells undergo apoptosis.Through lipofectin transinfection,we observed that only the DNA of AcMNPV or SfaMNPV can also induce apopotosis of SL-1 cell.Virus titer analysis revealed that neither viruses can duplicate in SL-1 cells.

关 键 词：杆状病毒 诱导  昆虫细胞凋亡  斜纹夜蛾细胞系  梯形带  

分 类 号：S476.13] Q965.8]