文献类型：期刊文章

作　　者：董志峰[1] 马荣才[2] 彭于发[3] 管华诗[1]

机构地区：[1]青岛海洋大学 [2]北京农业生物技术研究中心,北京100089 [3]中国农业科学院植物保护研究所,北京100094

出　　处：《Acta Botanica Sinica》

年　　份：2001

卷　　号：43

期　　号：7

起止页码：661-672

语　　种：中文

收录情况：CSCD、CSCD2011_2012、SCI-EXPANDED(收录号：WOS:000170245200001)、SCOPUS、WOS、普通刊

摘　　要：The biosafety of genetically engineered plants has been of concernment in society and science in recent years. The issue of 35S promoter of CaMV has been contentious because of its wide use in plant genetic engineering. The debate on the safety and potential risks of the 35S promoter will be discussed here. Some of concerns are expressed about the dissemination of antibiotic_resistance genes and vector backbone sequences. Various methods and strategies are currently being developed for the marker gene excision and elimination of vector backbone sequences from transgenic plants. In this review, the CRE/ lox system which could get rid of the marker geens and vector backbone sequences will be discussed in detail. Advances in the research of the safety assessment of genetically modified plants using the CRE/ lox system will also be described.

关 键 词：生物安全 35S启动子  标记基因 载体骨架序列  CRE/LOX 转基因植物 外源非目的基因  

分 类 号：Q943.2[植物生产类] S188]