文献类型：期刊文章

作　　者：魏东旺[1] 楼允东[1] 孙效文[2] 沈俊宝[2]

机构地区：[1]上海水产大学渔业学院,上海200090 [2]中国水产科学院黑龙江水产研究所,哈尔滨150070

出　　处：《Zoological Research》

基　　金：本研究为国家高技术发展计划资助项目 (课题编号 :10 1-0 5 -0 2 -0 1)

年　　份：2001

卷　　号：22

期　　号：3

起止页码：238-241

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、DOAJ、JST、PUBMED、SCOPUS、ZGKJHX、ZR、核心刊

摘　　要：A partial common carp genomic library was constructed.45 positive clones were isolated from screening about 2000 clones of the genomic library with a (CA) 15 probe labelled at the 5’ end with γ 32 P ATP.Sequencing of these clones was performed with automated sequencer,and 22 microsatellites were isolated.17 primers were designed based on unique sequences flanking each motif with the software Primer3.PCR on Cyprinus carpio haematopterus was carried out with these primers,and all gave expected bands.Annealing temperature of these primers was between 50℃ and 53℃.

关 键 词：鲤鱼 微卫星 分子标记 筛选  

分 类 号：Q75]