文献类型：期刊文章

作　　者：吴龙英[1]

机构地区：[1]贵州大学生物技术学院生物技术系,贵州贵阳550025

出　　处：《山地农业生物学报》

年　　份：2000

卷　　号：19

期　　号：3

起止页码：237-238

语　　种：中文

收录情况：ZGKJHX、普通刊

摘　　要：Using chitosan as a source, the chitosan microspheres were prepared by suspension crosslinking technique. Their properties were determined and analysed. The results indicated that they complexed functions of chitosan with those of macromolecule microspheres. Then as the carrier, the chitosan microspheres were used to immobilize papain by absorption crosslinking method. The optimum conditions for immobilization were studied. The results were as follows: the optimum load of enzyme was 1.92 mg enzyme/g carrier, the absorbing time was 12 h and the temperature 4～8 ℃; then interacted for 3 h at 4～8 ℃ with 0.5% glutaraldehyde. The activity and the activity recovery of the immobilized papain were 38.49 U/g carrier and 66.60% respectively. Key words:chitosan microspheres; papain;

关 键 词：沙金豆豉  产酱香芽孢杆菌  发酵食品

分 类 号：TS201.3] TS264.29[食品科学与工程类]