文献类型：期刊文章

作　　者：李华平[1] 胡晋生[2] 王敏[2] 范怀忠[1]

机构地区：[1]华南农业大学资环学院,广东广州510642 [2]夏威夷大学植病系,美国96822

出　　处：《病毒学报》

基　　金：教育部优秀年轻教师基金!( 2 0 0 0 11);广东省自然科学基金!( 990 714 )

年　　份：2000

卷　　号：16

期　　号：3

起止页码：276-278

语　　种：中文

收录情况：BDHX、BDHX1996、BIOSISPREVIEWS、CAB、CAS、CSCD、CSCD2011_2012、JST、PUBMED、SCOPUS、ZGKJHX、核心刊

摘　　要：The plant expression vector of the coat protein(CP) gene of cucumber mosaic virus (CMV) BS strain was used to transform three kinds of pepper (Capsicum annuum) tissues (cotyledon, stem and root) by agrobacterium-mediated co-cultivation. 53%-68.4% of the total tissues (639) can be induced to be calli, but only cotyledon calli can be further regenerated to form shoots (regenerated efficiency 39.7%). 70%(42/60) of the putative transformed plants were confirmed to have CP gene in their genomes by Southern blot. The mRNAs and the CP were respectively found in 80% of transgenic plants by Northern blot and DAS-ELISA. 24 of the transgenic plants expressing CP gene of BS strain showed three kinds of resistant level (severe symptom, delay of symptomatic development, no symptom) to infection of CMV-BS and of CMV-P. However, there was distinctly higher resistance to inoculation of CMV-BS than that with CMV-P in these transgenic plants.

关 键 词：黄瓜花叶病毒 辣椒 衣壳蛋白 表达  抗性

分 类 号：S432.41] S436.418.1]