文献类型：期刊文章

作　　者：袁丽佳[1] 王聪[1] 刘伟[1] 刘文龙[1] 苟宝迪[1] 张天蓝[1]

机构地区：[1]北京大学医学部化学生物学系,北京100191

出　　处：《Journal of Chinese Pharmaceutical Sciences》

基　　金：supported by the Project of National Base for Talent Training in Basic Science(Grant No.J0830836)

年　　份：2013

卷　　号：22

期　　号：2

起止页码：184-189

语　　种：中文

收录情况：CAB、CAS、CSCD、CSCD2013_2014、DOAJ、EMBASE、IC、JST、SCOPUS、ZGKJHX、普通刊

摘　　要：Realgar （As 4 S 4 ）, as a mineral drug in traditional Chinese medicine, is currently used as the remedy for acute promyelocytic leukemia and has been proven to have relatively milder side effects as compared to the arsenolite （As 2 O 3 ）-based drugs. We have previously demonstrated that realgar induces differentiation in HL-60 cells, and the differentiation is associated with serine/threonine protein phosphatases, MAPK signaling pathways, and mitochondrial transmembrane potential decrease. In this study, we further explore the roles of mitochondrial permeability transition pore and reactive oxygen species （ROS） in realgar-induced differentiation in HL-60 cells. The differentiation was preceded by marked changes in the cellular level of ROS, and could be enhanced by SB202190, a p38 MAPK inhibitor. In addition, the efficacy of realgar was suppressed by closing the MPTP with an inhibitor. Taken together, these findings indicate that the opening of MPTP and the alteration of ROS generation were involved in realgar-induced differentiation.

关 键 词：Realgar Cell differentiation Reactive oxygen species Mitochondrial permeability transition pore HL-60 cells  

分 类 号：R285[中药学类]