期刊文章详细信息
Isolation and Identification of Glyphosate-degraded Strain Aspergillus oryzae sp.A-F02 and Its Degradation Characteristics
草甘膦降解菌A-F02的分离·鉴定及降解特性研究(英文)
文献类型:期刊文章
机构地区:[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学中德食品工程中心,江西南昌330047 [3]南昌大学生命科学与食品工程学院食品工程系,江西南昌330031 [4]南昌大学生命科学与食品工程学院生物技术系,江西南昌330031
出 处:《Plant Diseases and Pests》
年 份:2010
卷 号:1
期 号:5
起止页码:54-57
语 种:中文
收录情况:CAB、CAS、CSA、PROQUEST、外刊、普通刊
摘 要:[Objective] The research aimed to isolate the glyphosate-degraded strain and study its degradation characteristics.[Method] A glyphosate-degraded fungal strain A-F02 was isolated from sludge in an aeration tank of a glyphosate manufacture.The fungal strain A-F02 was identified according to morphological characteristics and internal transcribed spacer(ITS)region of nuclear ribosomal DNA sequence analysis.The glyphosate-biodegraded characteristics of strain A-F02 and the influencing factors were studied.[Result] The fungal strain A-F02 was identified as Aspergillus oryzae sp..The glyphosate-biodegraded rate was 86.82% in the mineral salt medium with 1 000 mg/L of glyphosate as the sole source of carbon,after being incubated at 30 ℃ and 150 rpm for 7 d.The biodegradation rates and biomass of the A-F02 were the highest under the culture conditions with glucose(0.5%,w/v),pH 7.5,30 ℃ and glyphosate(1 500 mg/L).[Conclusion] The research provided the experimental basis for glyphosate-biodegraded enzyme purification.
关 键 词:lyphosate-biodegraded rate BIOMASS Aspergillus oryzae sp. A-F02
分 类 号:X172]
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