文献类型：期刊文章

作　　者：王毅[1] 王本祥[1] 刘铁汉[2] 南陆彦[3] 永田年[4] 池岛乔[1]

机构地区：[1]长春中医学院新药研究中心 [2]沈阳药科大学 [3]横滨市立大学医学部寄生虫教研室 [4]滨松医科大学微生物教研室

出　　处：《Acta Pharmacologica Sinica》

基　　金：Project supported by the National Natural Science Foundation of China, No 395708612.

年　　份：2000

卷　　号：21

期　　号：9

起止页码：26-30

语　　种：中文

收录情况：AJ、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、FSTA、IC、JST、PROQUEST、PUBMED、SCIE、SCOPUS、WOS、ZGKJHX、普通刊

摘　　要：AIM: To compare the effect of ginsenoside Rg1 and its metabolite Rh, on proinflammatory cytokines and their mRNA expression by THP-1 cells. METHODS: Hu-man peripheral blood mononuclear cells (PBMC) were incubated with Rg1 and Rh1 at concentrations of 0.1, 1, 10, and 100 mg/L, and the cell proliferation was mea-sured 24 h after incubation. Radioimmunoassay (RIA) was used to detect the production of proinflammatory cy-tokines, TNFα, IL-1α, and IL-8. TNFα mRNA level was detected by reverse transcription polymerase chain re-action (RT-PCR) after administration of Rg1 and Rh1. RESULTS: Rg1 and Rh1(at concentration of 0.1, l, 10, 100 mg/L) had no effect on PBMC proliferation. Rh1 1 mg/L could upregulate the productions of TNF (and IL-8 induced by lipopolysaccharides (LPS) 10 mg/ L plus phorbol myristate acetate (PMA) 200 nmol/L, however, Rg1 showed an inhibitory effect on TNFα pro-duction induced by LPS 100 mg/L. Rg1 1 mg/L and Rh1, 100 mg/L enhanced the production of IL-lα level in THP-1 cells in the presence of LPS 10 mg/L. RT-PCR revealed that Rh1 stimulated TNFα mRNA expression in suitable stimulatory conditions. CONCLUSION: Rg1 and Rh1 have different effects on the production of cy-tokines produced THP-1 cells stimulated by LPS and PMA.

关 键 词：GINSENG SAPONINS CYTOKINES RADIOIMMUNOASSAY reverse transcriptase polymerase chain reaction  

分 类 号：R285[中药学类]