文献类型：期刊文章

作　　者：李英[1] 许文燮[1] 李在琉[1]

机构地区：[1]延边大学医学院消化生理研究室

出　　处：《Acta Pharmacologica Sinica》

基　　金：Project supported by the National Natural Science Foundation of China, № 39660026

年　　份：2000

卷　　号：21

期　　号：6

起止页码：93-98

语　　种：中文

收录情况：AJ、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、FSTA、IC、JST、PROQUEST、PUBMED、SCI-EXPANDED(收录号：WOS:000087561300014)、SCIE、SCOPUS、WOS、ZGKJHX、普通刊

摘　　要：AIM:To determine the effect of nitric oxide (NO) on calcium-activated potassium currents in antral circular my-ocytes of the guinea pig stomach. METHODS: The whole-cell patch-clamp techniques were used, and the cells were isolated by collagenase. Sodium nitroprusside (SNP), spermine, and 3-morpholino-sydnonimine (SIN-1) were used as nitric oxide donors. RESULTS: Outward potassium currents were remarkably inhibited by te-traethylammonium (TEA) 1 mmol·L-1. Charybdotoxin 200 nmol·L-1, a specific inhibitor of KCa, channel, also greatly inhibited IK(Ca) · NO donors remarkably increased IK(Ca) in guinea pig gastric antral circular my-ocytes with perforated patch-clamp techniques but not conventional whole-cell patch-clamp techniques. Spermine 100 μmol · L-1 increased IK(Ca) by 14 % ± 10 % (at 60 mV, P<0.01), SIN-1 200 μmol·L-1 increased IK(Ca) by 19 % ± 14 % (at 60 mV, P <0.01) , and SNP 100 μmol·L-1 increased IK(Ca) by 24 % ± 13 %(at 60 mV, P<0.01) respectively. SIN-1- and SNP-in-duced increase of IK(Ca) was blocked by methylene blue 1 μmol· L -1. CONCLUSION: NO increases calcium-activated potassium currents in gastric antral circular my-ocytes of guinea pig, and the effect of NO on IK(Ca) be mediated by cyclic GMP.

关 键 词：pyloric antrum  patch-clamp techniques  NITROPRUSSIDE sydnones  SPERMINE tetraethylam- monium  methylene blue  

分 类 号：R96]