文献类型：期刊文章

作　　者：施玉樑[1] 白俊平[1] 王文萍[1]

机构地区：[1]中国科学院上海生命科学研究院神经生物学重点实验室

出　　处：《Acta Pharmacologica Sinica》

基　　金：Project supported by the National Natural Science Foundation of China (№ 38388013, 38970831, 39480005, and 39870197).

年　　份：2003

卷　　号：24

期　　号：1

起止页码：24-32

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、FSTA、IC、JST、PROQUEST、PUBMED、SCI-EXPANDED(收录号：WOS:000180305400003)、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：Ion channel plays a key role in maturation, capacitation and acrosome reaction of sperm. However, as it is difficult to record channel currents from a mature mammal sperm directly by patch-clamp technique, there were no basic data on the types and properties of the channels in human sperm until the method reconstituting the channels into bilayer was used. By reconstituting the channel proteins isolated from sperm membrane into phospholipidforming bilayer, we have characterized several kinds of Ca^(2+), Na^+-, K^+-, and Cl^--permeable channels with different conductance and properties in human sperm membrane. To study the channels in spermatogenic cells is another approach to understand the ion-channels in mature sperm. The cell is used as a model to analyze the effects of male antifertility agents on Ca^(2+)-channel. To date, several male contraceptives derived from Chinese traditional medicine have been attached worldwide interest, a lot of compounds have been purified from them, and the antifertility effects of some compounds were demonstrated. We studied the effects of gossypol and several compounds isolated from Tripterygium wilffordii on Ca^(2+) channel in mouse spermatogenic cells and found that each of them inhibited the channel and sperm acrosome reaction at a proximate concentration, suggesting that the inhibition of Ca^(2+) channels may be one mechanism of the antifertility effects of these contraceptives.

关 键 词：SPERMATOZOA ion channels  lipid bilayers  spermatogenic cells  male antifertility agents  Chinese herbal medicine  

分 类 号：R285[中药学类]