文献类型：期刊文章

作　　者：孙朝霞[1] 侯思宇[2] 王玉国[1]

机构地区：[1]山西农业大学农学院生物技术系,山西太谷030801 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《Agricultural Science & Technology》

年　　份：2008

卷　　号：9

期　　号：3

起止页码：46-48

语　　种：中文

收录情况：CAB、CAS、CSA、CSA-PROQEUST、PROQUEST、普通刊

摘　　要：[Objective] The study aimed to reveal the effect of osmosis-regulating substances and organic appendices on somatic embryogenesis in wheat. [Method] The suitable concentration combination of appendices was optimized by adding different concentrations of osmosis-regulating substances including mannitol, sorbitol and organic appendices such as Gln, CH and LH, into the somatic embryogenesis in wheat. [Result] The mannitol or sorbitol lower than 40 g/L was helpful for improving somatic embryogenesis; there was no significant difference in the induction rate of somatic embryogenesis when 300-500 mg/L Gln、CH or LH was respectively added into the induced medium, while somatic embryogenesis could be enhanced dramatically in the presence of 500 mg/L Gln together with 300 mg/L CH. [Conclusion] Somatic embryogenesis could be improved to some extent by different concentrations of osmosis-regulating substances and organic appendices, which laid foundation for establishing a more perfect system of somatic embryogenesis in wheat.

关 键 词：WHEAT SOMATIC EMBRYOGENESIS ORGANIC appendix  Osmosis-regulating  substance  

分 类 号：S512.1]