文献类型：期刊文章

作　　者：张承悦[1] 赵攀[1] 施喆[1] 杨晓达[1]

机构地区：[1]北京大学医学部天然药物及仿生药物国家重点实验室药学院化学生物学系,北京100191

出　　处：《Journal of Chinese Pharmaceutical Sciences》

基　　金：Major New Medicine Project in Mega-projects of Science Research of China(Grant No.2009ZX09502-006);National Natural Science Foundation of China(Grant No.20971008)

年　　份：2011

卷　　号：20

期　　号：6

起止页码：584-589

语　　种：中文

收录情况：CAB、CAS、DOAJ、EMBASE、IC、JST、SCOPUS、ZGKJHX、普通刊

摘　　要：inducing resuscitation with herbal aromatics is important to modulate the brain intake of drugs in traditional Chinese medicine,but limited information has been available on the mechanism of action.The MDCK-MDRl monolayer is an excellent in vitro cell model to use as a tool to study blood brain barrier（BBB） screening.In this study,we established MDCK-MDR1 cell line by stable transfection and investigated the effects of several important herbal aromatics on BBB permeability.The characterization experiment demonstrated the MDCK-MDRl used in this study was valid.In a transport study,we found several herbal aromatics increased the permeability of fluorescein isothiocyanate-labeled dextran 4kDa（FD4） and inhibited efflux of Rhodaminel23（Rhol23）.These results demonstrated that herbal aromatics enhanced the BBB permeation of drugs by both inhibition of P-gp and opening of the BBB tight junction,thus providing new insights for understanding the mechanisms of aromatic compounds＇ BBB permeability.

关 键 词：Herbal aromatics  MDCK-MDR1  Tight junction  P-GP

分 类 号：R285[中药学类]