文献类型：期刊文章

作　　者：梁婧娴[1] 陈志成[1] 郑玉才[1] 杨虎林[2] 徐亚欧[1]

机构地区：[1]西南民族大学,四川成都610041 [2]四川省阿坝州阿坝县畜牧局,四川阿坝624600

出　　处：《Agricultural Science & Technology》

基　　金：Supported by Key Project of Applied Basic Research of Sichuan Province(07JY029-045)~~

年　　份：2011

卷　　号：12

期　　号：4

起止页码：608-612

语　　种：中文

收录情况：CAB、CAS、CSA、CSA-PROQEUST、PROQUEST、普通刊

摘　　要：[Objective] The aim was to investigate the difference in MSTN gene expression in different tissues of Tibetan sheep at different ages.[Method] According to the sequence（NM_001009428.1）published in GenBank,a pair of specific primers was designed to amplify part of cDNA sequence of MSTN by using QRT-PCR technique.The relative expression level of MSTN gene in rennet stomach,rumen,leg muscle and cardiac muscle of Tibetan sheep at different ages were analyzed.[Result] After normalization with β-actin gene,the relative expression level of MSTN gene in the 6-month-old Tibetan sheep was the highest and it was 2.52 times than that in 12-month-old Tibetan sheep（P0.05）,the relative expression level of MSTN gene in leg muscle was the highest among all tissues and it was 3 984.78 times than that in rumen（P0.01）.[Conclusion] The results established theoretical foundation for the correct use of MSTN antibody.

关 键 词：Tibetan sheep  Myostatin（MSTN）  Real-time PCR  

分 类 号：S826]