文献类型：期刊文章

作　　者：王斌[1,2] 李传友[1,2] 郑洪刚[1,2] 伏健民[1,2] 杨仁崔[1,2] HenryT.NGUYEN[1,2]

机构地区：[1]中国科学院遗传研究所 [2]福建农业大学作物遗传育种研究所

出　　处：《Acta Botanica Sinica》

年　　份：1999

卷　　号：41

期　　号：5

起止页码：502-507

语　　种：中文

收录情况：CSCD、CSCD2011_2012、SCOPUS、普通刊

摘　　要：AFLP analysis was performed between a pair of thermo_sensitive genic male sterile (TGMS) rice allelic mutant lines (5460S and 5460F). The reaction conditions for rice AFLP assay were optimized. The relative efficiencies for polymorphism detection of RFLP, RAPD and AFLP were compared. The results indicated that the efficiency for polymorphism detection in rice was in the order of AFLP>RAPD>RFLP, and also indicated that AFLP was a powerful DNA molecular marker technique for polymorphism detection, especially in the case of extremely low polymorphism, such as isogenic lines and allelic mutant lines. Some of the AFLP products between the TGMS rice allelic mutant lines were cloned. Three of them were used as mixed probes to screen BAC library of rice line 5460S. 12 positive clones were screened out. In addition, the advantages and disadvantages of these three molecular marker systems were discussed.

关 键 词：AFLP RICE Molecular marker  BAC library screening  

分 类 号：S511.01]