文献类型：期刊文章

作　　者：周瑞雪[1,2] 蒙涛[1,2] 孟海波[1] 陈敦学[1] 宾石玉[2] 成嘉[1] 符贵红[1] 褚武英[1] 张建社[1]

机构地区：[1]长沙大学生物工程与环境科学系,湖南长沙410003 [2]广西师范大学生命科学学院,广西桂林541004

出　　处：《Zoological Research》

基　　金：国家自然科学基金(30771644;30972263);Aid Program for Science and Technology Innovative Research Team in Higher Educational Instituions of Hunan Province

年　　份：2010

卷　　号：31

期　　号：2

起止页码：141-146

语　　种：中文

收录情况：AJ、BDHX、BDHX2008、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、DOAJ、JST、PUBMED、SCOPUS、ZGKJHX、ZR、核心刊

摘　　要：At present, transcription analysis of gene expression commonly uses housekeeping genes as control for normalization. In this study, the expression levels of three housekeeping genes including GAPDH, β-actin, and 18S rRNA in six tissues and five developmental stages of the Mandarin fish Siniperca chuatsi were assayed with quantitative real-time PCR （qPCR）. Differences in expression levels were analyzed using geNorm program. The results demonstrate that β-actin is the most stable gene at developmental stages and GAPDH is the most stable in different tissues. While 18S rRNA expression during development is differentially regulated, which indicates it is suitable as an internal control for gene expression normalization at the developmental level. Overall, the data suggest that the two most stable housekeeping genes are enough to accurately calibrate gene expression in S. chuatsi. The significance of this study provided convincing references and methodology for housekeeping gene selection and normalization in gene expression analysis with regular PCR or qPCR.

关 键 词：Reference genes  geNorm program  Gene expression  Real-time PCR  

分 类 号：Q953]