文献类型：期刊文章

作　　者：朱壮春[1] 陈阳[1] 郝东升[2] 王艳茹[1] 马玉妹[1] 曹向可[1] 穆艮艮[1] 李金坤[1] 史相国[3]

机构地区：[1]华北煤炭医学院生物科学系,河北唐山063000 [2]华北煤炭医学院附属医院,河北唐山063000 [3]唐山陡河水库管理处,河北唐山063000

出　　处：《Agricultural Science & Technology》

基　　金：Supported by Science and Technology Key Project of Science and Technology Bureau of Tangshan:Study of Rapid Detection and Diag-nosis Technology on Key Aquatic Animal Pathogen(06125401A-3)~~

年　　份：2009

卷　　号：10

期　　号：5

起止页码：116-118

语　　种：中文

收录情况：CAB、CAS、CSA、CSA-PROQEUST、PROQUEST、普通刊

摘　　要：[Objective] The study aimed to establish an approach to high titer Et immune serum preparation by low-aged Japanese rabbits. [Method] Antigen of Et was prepared at first, Japanese rabbits were taken as immunized animal, and divided into two groups for experiments, one is two-month-old group （T group）, and another is six-month-old group （S group）. Japanese rabbits were continuous by immunized with low-dose by using auricular intravenous method, then immune sera were collected. Immune serum antibody titer was determined with micro-agglutination reaction method. E Resultl Agglutination reaction showed that the Et serum titer of S group is higher than that of T group in the first testing with the same dose. But in the second testing, the serum titer of the T group and and the S group was consistent, [Conclusien] The animals in the T group （Japanese rabbits） were fewer months old, and produced high titer antiserum was which consistent with the S group, which indicated that the method of preparing of high titer Et immune serum by low-aged Japanese rabbits was feasible.

关 键 词：Edwardsiella tarda  IMMUNITY Immune serum  Japanese rabbit  

分 类 号：S852.5]