文献类型：期刊文章

作　　者：曹红[1] 崔一卉[2] 赵志奇[1] 曹晓华[2] 张玉秋[1]

机构地区：[1]复旦大学脑科学研究院,医学神经生物学国家重点实验室,神经生物研究所,上海200032 [2]华东师范大学脑功能基因组学教育部重点实验室,上海市科学技术委员会重点实验室,上海200062

出　　处：《Neuroscience Bulletin》

基　　金：supported by National Natural Science Fundation of China (No.30870835,30821002,and 30900444);National Basic Research Program of China (No. 2007CB512303,2007CB512502,and 2006CB500807);Postdoctoral Fundation of China (No.20080440578)

年　　份：2009

卷　　号：25

期　　号：5

起止页码：301-308

语　　种：中文

收录情况：CAS、CSCD、CSCD2011_2012、EBSCO、EMBASE、IC、JST、PUBMED、SCI(收录号：WOS:000208089300008)、SCI-EXPANDED(收录号：WOS:000208089300008)、SCIE、SCOPUS、WOS、ZGKJHX、普通刊

摘　　要：Objective To explore the role of the extracellular signal-regulated kinase （ERK）/cAMP response element binding protein （CREB） pathway in the induction of long-term potentiation （LTP） in the anterior cingulate cortex （ACC） that may be implicated in pain-related negative emotion. Methods LTP of field potential was recorded in ACC slice and the expressions of phospho-ERK （pERK） and phospho-CREB （pCREB） were examined using immunohistochemistry method. Results LTP could be induced stably in ACC slice by high frequency stimulation （2-train, 100 Hz, 1 s）, while APv （an antagonist of NMDA receptor） could block the induction of LTP in the ACC, indicating that LTP in this experiment was NMDA receptor-dependent. Bath application of PD98059 （50 μmol/L）, a selective MEK inhibitor, at 30 min before tetanic stimulation could completely block the induction of LTP. Moreover, the protein level of pERK in the ACC was transiently increased after LTP induction, starting at 5 rain and returning to basal at 1 h after tetanic stimulation. The protein level of pCREB was also increased after LTP induction. The up-regulation in pERK and pCREB expressions could be blocked by pretreatment of PD98059. Double immunostaining showed that after LTP induction, most pERK was co-localized with pCREB. Conclusion NMDA receptor and ERK-CREB pathway are necessary for the induction of LTP in rat ACC and may play important roles in pain emotion.

关 键 词：long-term potentiation  extracellular signal-regulated kinase  cAMP response element binding protein  anterior cingulate cortex  RAT

分 类 号：R338.3] Q432[基础医学类]