文献类型：期刊文章

作　　者：张业顺[1,2] 张国政[1,2] 韦亚东[2] 夏定国[2]

机构地区：[1]江苏科技大学,江苏镇江212003 [2]中国农业科学院农业部蚕桑遗传改良重点实验室,江苏镇江212018

出　　处：《Agricultural Science & Technology》

年　　份：2009

卷　　号：10

期　　号：4

起止页码：167-170

语　　种：中文

收录情况：CAB、CAS、CSA、CSA-PROQEUST、PROQUEST、普通刊

摘　　要：[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extruding and centrifugal method, and then the semen was injected into other virgin moths with trypsinase. [ Result] A high-effective collection technology of spermatids from silkworm was established successfully, 50 μl semen could be collected by only one person in each hour. The survival rate of spermatids was over 80% in vito after collected from bursa copulatrix, while the obtained semen was quite pure and the average fertilization rate of silkworm was 76,5%. [ Conclusion] The establishment of high-effective semen extraction technique of silkworm provides the technical basis for studies on other related techniques for silkworm sperm.

关 键 词：Bombyx mori  Artificial insemination  Key technique  

分 类 号：S882]