文献类型：期刊文章

作　　者：刘欣[1] 马小琛[1] 黄和[1,2] 于文涛[1] 纪晓俊[1] 彭超[1] 高振[1]

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009 [2]江苏省工业生物技术创新中心,江苏南京211816

出　　处：《Agricultural Science & Technology》

基　　金：Supported by the National Natural Science Foundation of China(20606018);the National Basic Research Program (also called 973Program)(2007CB707805);the Six Projects Sponsoring Talent Summits of Jiangsu Province~~

年　　份：2009

卷　　号：10

期　　号：4

起止页码：12-14

语　　种：中文

收录情况：CAB、CAS、CSA、CSA-PROQEUST、PROQUEST、普通刊

摘　　要：[ Objective] The study was to investigate the volatile components of secondary metabolites from M. alpine producing arachidonic acid and explore the changes in its metabolic pathway. [ Method] The air above M. alpine broth was extracted by solid-phase microextraction（SPME） during the post-exponential phase of growth and analyzed by GC-MS. [Result] 13 compounds were identified, 12 of which were sesquiterpenes with C15H24 formula and accounting for 99.62% of the complete compounds. Thujopsene-（ 12）, α-Guaiene and Aristolene were three most sesquiterpenes accounting for 10.66%, 33.69% and 34.85% of total content respectively. It can be sufficiently certified that sesquiterpene metabolic pathway existing in M. alpine. [ Coclusion] Metabolic flux of sesquiterpene pathway increased to improve its mass accumulation, because one or several key enzyme genes mutation in isoprene or sesquiterpene pathway enhanced their activities during induction of mutation from initial strain.

关 键 词：Mortierella alpina  SPME SESQUITERPENE Arachidonic acid  

分 类 号：S182]