文献类型：期刊文章

作　　者：罗燕[1] 程建国[2] 郑士华[1] 赵翠[1] 李蓓[1] 李敏[1]

机构地区：[1]四川农业大学都江堰分校生物科学系,四川都江堰611830 [2]四川养麝研究所,四川都江堰611830

出　　处：《Agricultural Science & Technology》

基　　金：Supported by Youth Foundation of Education Department in Sichuan Province (07ZB060);Youth Science and Technology Innovation Fund in Sichuan Agricultural University~~

年　　份：2009

卷　　号：10

期　　号：3

起止页码：22-25

语　　种：中文

收录情况：CAB、CAS、CSA、CSA-PROQEUST、PROQUEST、普通刊

摘　　要：[Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. [Method] Plasmids from 24 pathogenic Escherichia coli in musk deer were extracted by the Lysis Triton method, and then identified by single enzyme digestion with three endonucleases of Hind Ⅲ, EcoR Ⅰ and BamH Ⅰ. [Result] The yield rate of plasmids was 91.6%, and 24 pathogenic Escherichia coli in musk deer had the identical or similar plasmid profiles. [Conclusion] Plasmid DNA analysis offers scientific basis for molecular epidemiology of pathogenic Escherichia coli in musk deer in Sichuan Institute of Musk Deer Breeding.

关 键 词：Musk deer  Pathogenic Escherichina coil  Plasmid DNA Plasmid profile  

分 类 号：S852.61]