文献类型：期刊文章

作　　者：张俊平 张珉[1] 周俭平[1] 刘福堂[1] 周斌[1] 谢渭芬[2] 郭澄[2] 张纯[2] 钱定华[1]

机构地区：[1]第二军医大学药学院生化药学教研室 [2]长征医院,上海中国200433

出　　处：《Acta Pharmacologica Sinica》

基　　金：Project supported by the National Natrual Science Foundation of China, № 39670837.

年　　份：2001

卷　　号：22

期　　号：2

起止页码：91-94

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、FSTA、IC、JST、PROQUEST、PUBMED、SCI-EXPANDED(收录号：WOS:000166900000015)、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：AIM: To study the antifibrotic effects of matrine in vitro and in vivo. METHODS: Rat hepatic stellate cell HSC-T6 and mouse fibroblast cell NIH3T3 proliferation stimulated with serum and platelet-derived growth factor (PDGF) was measured by crystal violet staining assay. Collagen synthesis stimulated with serum and transforming growth factor β1(TGF-β1) was determined by [3 H] pro-line incorporation. Liver fibrosis was induced by carbon tetrachloride (CCl4) in rats and evaluated with plasma hyaluranic acid level and hepatic hydroxyproline content. RESULTS: Matrine (1-2 mmol·L-1) markedly reduced serum-driven proliferation and collagen synthesis of HSC-T6 cells as well as NIH3T3 cells. PDGF-driven proliferative activity and TGF-β1-driven collagen synthesis in HSC-T6 cells were attenuated by matrine (0. 25 - 2 rnmol·L-1) in a concentration-dependent manner. In vivo matrine (50 mg·kg-1 and 100 mg·kg-1) significantly decreased serum hyaluranic acid levels and hepatic hydroxyproline contents in rats treated with CCl4. CONCLUSION: Inhibition of PDGF and TGF-β1 actions on hepatic stellate cell by matrine might provide a possible mechanism of its antifibrotic activities.

关 键 词：MATRINE liver cirrhosis  cell division  COLLAGEN platelet-derived growth factor  transforming growth factor beta  

分 类 号：R285[中药学类]