期刊文章详细信息
Increment of calcium-activated and delayed rectifier potassium current by hyposmotic swelling in gastric antral circular myocytes of guinea pig
低渗膨胀增加豚鼠胃窦环行肌细胞钙激活钾电流和延迟整流型钾电流
文献类型:期刊文章
机构地区:[1]延边大学医学院消化生理研究室,延吉中国133000
出 处:《Acta Pharmacologica Sinica》
基 金:National Natural Science Foundation of China,No 39860031
年 份:2001
卷 号:22
期 号:6
起止页码:90-96
语 种:中文
收录情况:AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、FSTA、IC、JST、PROQUEST、PUBMED、SCI-EXPANDED(收录号:WOS:000169174700016)、SCIE、SCOPUS、WOS、ZGKJHX、核心刊
摘 要:AIM: To observe the effect of hyposmotic swelling on calcium-activated potassium current [ IK(ca) ] and delayed rectifier potassium current [IK(v) ] in gastric antral circular myocytes of guinea pig. METHODS: The whole cell patch-clamp technique was used, and the myocytes were isolated by collagenase. Cells were swelled by the hyposmotic solution (200 Osmmol/kg). RESULTS: The hyposmotic solution markedly increased IK(ca) and IK(v). The increase of IK(ca) was markedly inhibited by tetrathylammonium (TEA) 4 mmol·L-1 and charybdo-toxin (ChTX) 200 nmol·L-1. The increase of IK(V) was incompletely blocked by TEA and completely blocked by 4-aminopyridine (4-AP) 10 mmol · L-1. There was no significant difference between the amplitudes of the increase of IK(ca) and IK(V) ( P > 0.05). IK(ca) increased (17.0±4.8) s after the cells were perfused with the hyposmotic solution, whereas IK(V) increased (30.7 ±13.7) s after the cells exposed to the hyposmotic solution. There was significant difference between the latency of IK(ca) and IK(V) (P < 0.05). CONCLUSION: Hyposmotic swelling increased both IK(ca) and IK(v) , and the increment was likely related to the cell volume regulation.
关 键 词:potassium channels pyloric antrum guinea pigs patch-clamp techniques
分 类 号:R333]
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