文献类型：期刊文章

作　　者：朴琳[1] 李英[1] 李林[1] 许文燮[1]

机构地区：[1]延边大学医学院消化生理研究室,延吉中国133000

出　　处：《Acta Pharmacologica Sinica》

基　　金：National Natural Science Foundation of China,No 39860031

年　　份：2001

卷　　号：22

期　　号：6

起止页码：90-96

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、FSTA、IC、JST、PROQUEST、PUBMED、SCI-EXPANDED(收录号：WOS:000169174700016)、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：AIM: To observe the effect of hyposmotic swelling on calcium-activated potassium current [ IK(ca) ] and delayed rectifier potassium current [IK(v) ] in gastric antral circular myocytes of guinea pig. METHODS: The whole cell patch-clamp technique was used, and the myocytes were isolated by collagenase. Cells were swelled by the hyposmotic solution (200 Osmmol/kg). RESULTS: The hyposmotic solution markedly increased IK(ca) and IK(v). The increase of IK(ca) was markedly inhibited by tetrathylammonium (TEA) 4 mmol·L-1 and charybdo-toxin (ChTX) 200 nmol·L-1. The increase of IK(V) was incompletely blocked by TEA and completely blocked by 4-aminopyridine (4-AP) 10 mmol · L-1. There was no significant difference between the amplitudes of the increase of IK(ca) and IK(V) ( P > 0.05). IK(ca) increased (17.0±4.8) s after the cells were perfused with the hyposmotic solution, whereas IK(V) increased (30.7 ±13.7) s after the cells exposed to the hyposmotic solution. There was significant difference between the latency of IK(ca) and IK(V) (P < 0.05). CONCLUSION: Hyposmotic swelling increased both IK(ca) and IK(v) , and the increment was likely related to the cell volume regulation.

关 键 词：potassium channels  pyloric antrum  guinea pigs  patch-clamp techniques  

分 类 号：R333]