文献类型：期刊文章

作　　者：闫苗苗[1] 魏光成[1] 潘效红[1] 马怀雷[1] 李伟振[1]

机构地区：[1]滨州医学院烟台校区

出　　处：《Agricultural Science & Technology》

年　　份：2008

卷　　号：9

期　　号：2

起止页码：39-41

语　　种：中文

收录情况：CAB、CAS、CSA、CSA-PROQEUST、PROQUEST、普通刊

摘　　要：[Objective] The study aimed to introduce a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. [ Method ] The genomic DNAs were extracted from tender leaves of 24 peanut cuhivars and from the liver, lung and kidney of white mouse through the specifically modified CTAB method. The DNAs were run on agarose gel, next detected by DNA/Protein analyzer. Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method. [ Result] The clear and orderly bands were observed in gel detection, and the values of OD200/OD200 for DNAs extracted via modified CTAB method were between 1.77 - 1.83. The DNAs performed well in PCR amplification. [ Conclusion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification.

关 键 词：ANIMAL PLANT Extraction of genomic DNA  Modified CTAB method  

分 类 号：Q943.2[植物生产类]