文献类型：期刊文章

作　　者：杨玲玲[1] 朱少彬[1] 杭纬[1] 颜晓梅[1]

机构地区：[1]厦门大学化学化工学院化学生物学系 化学生物学福建省重点实验室 现代分析科学教育部重点实验室,厦门361005

出　　处：《高等学校化学学报》

基　　金：国家自然科学基金(批准号:20645001,20675070);福建省科技厅科研基金(批准号:2005NZ1013);教育部留学回国人员科研启动基金;2006年度福建省新世纪优秀人才支持计划;2007年度教育部新世纪优秀人才支持计划基金资助

年　　份：2008

卷　　号：29

期　　号：8

起止页码：1549-1551

语　　种：中文

收录情况：AJ、BDHX、BDHX2004、CAS、CSCD、CSCD2011_2012、EI(收录号：20084411669029)、IC、JST、RCCSE、RSC、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：A single-molecule flow analyzer based on laser induced fluorescence and hydrodynamic focusing was developed in our lab. There were three key aspects of this design for achieving single molecule detection sensitivity: (1) a detector with a high photon-detection efficiency(>65% for avalanche photo-diode detector); (2) a small probe volume obtained by sheath-protected, hydrodynamically focused sample stream to reduce background; and (3) a slow flow to provide extended dwell time(milliseconds) in the probe volume so that more fluorescence cycling can occur. The single molecule detection sensitivity was demonstrated by the successful detection of single phycoerythrin molecules. The house-constructed single-molecule flow analyzer was applied to DNA fragment sizing with linear correlation between DNA fragment length and mean burst size.

关 键 词：单分子检测 流体聚焦  藻红蛋白

分 类 号：O657.38]