文献类型：期刊文章

作　　者：姚倩[1] 侯世祥[2] 何希辉[3] 张瑄[2] 颜军[1] 苟小军[1]

机构地区：[1]成都大学四川省药用微生物资源重点实验室,四川成都610106 [2]四川大学华西药学院药剂学系,四川成都610041 [3]北京大学药学院化学生物学系,北京100083

出　　处：《Journal of Chinese Pharmaceutical Sciences》

基　　金：Postdoctoral Scientific Research Station of Gansu Yasheng Groups.

年　　份：2008

卷　　号：17

期　　号：2

起止页码：158-162

语　　种：中文

收录情况：CAB、CAS、DOAJ、EMBASE、IC、JST、SCOPUS、ZGKJHX、普通刊

摘　　要：To develop a sensitive high performance liquid chromatography （HPLC） assay for the determination of trans-resveratrol in mouse liver. The whole liver of a mouse was removed from the body, homogenated, and extracted by ethyl acetate. The organic layer was isolated and evaporated to dryness, the residue was reconstituted in 0.2 mL mobile phase for centrifugation, and 50 uL of the supernatant was injected into the/-IPLC instrument. The sample was separated on a Shimadzu ODS column （150 mm × 4.6 mm, 5 um） at 35 ℃ and detected by ultraviolet （UV） detector at the wavelength of 305 nm. The mobile phase consisted of methanol and 0.1 mol/L acetic acid （4：6, v/v） with the flow-rote at 1 mL/min. The limit of detection was 3.0 ng/g in liver homogenate with a signal/noise ratio of 3：1. The linear range of the calibration curve was 5.0-120.0 ng/g. The mean recoveries at the concentrations of 6, 10 and 80 ng/g were 102%, 96.0% and 91.5%, respectively. The RSDs for inter- and intra-day assays were less than 5%. Compared with other reported methods, this method was faster and more sensitive. It was also proved to be of good linearity, selectivity, accuracy and precision, and can be efficiently applied to the pharmacoldnetic study of trans-resveratrol in mouse liver.

关 键 词：High performance liquid chromatography  TRANS-RESVERATROL Mouse liver  

分 类 号：R285[中药学类]