文献类型：期刊文章

作　　者：费砚群[1] 罗贵民[1] 冯国栋[2] 费强[2] 陈焕文[2] 梁学凯[2] 赵晓君[3] 郇延富[1,2]

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春130021 [2]吉林大学化学学院,长春130012 [3]北达科他大学化学系,大福克斯58203

出　　处：《高等学校化学学报》

基　　金：中国博士后科学基金(批准号:20040350561)资助

年　　份：2008

卷　　号：29

期　　号：1

起止页码：83-85

语　　种：中文

收录情况：AJ、BDHX、BDHX2004、CAS、CSCD、CSCD2011_2012、EI(收录号：20081711223291)、IC、JST、RCCSE、RSC、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET).Nanoparticle A was doped with a dye complex which consisted of two dye molecules,fluorescein and tetramethylrhodamine.These dye molecules were first linked with avidin and biotin,respectively.They then formed a stable dye complex through avidin-biotin bridge within the distance of FRET.Nanoparticle B was doped with fluorescein only.As a result of this combination,the nanoparticles emit double wavelengths when a single excitation wavelength is used.The results show that the fluorescent nanoparticles are promising fluorescent labeling reagents for the sensitive detection of double targets.

关 键 词：纳米粒子  双重对象  同时检测  荧光共振能量转移

分 类 号：O657.34]