文献类型：期刊文章

作　　者：张彤晴[1] 杨洪生[2] 林海[1] 王静[2] 周刚[1] 李强[3]

机构地区：[1]江苏省淡水水产研究所,江苏南京210017 [2]江苏省水产质量检测中心,江苏南京210017 [3]扬州大学,江苏扬州225009

出　　处：《水产学报》

基　　金：江苏省科技厅高新技术研究项目(BG2005324);国家"十一五"科技支撑计划(2006BAD03B07)

年　　份：2007

卷　　号：31

期　　号：5

起止页码：699-703

语　　种：中文

收录情况：AJ、BDHX、BDHX2004、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、IC、JST、PROQUEST、RCCSE、SCOPUS、WOS、ZGKJHX、ZR、核心刊

摘　　要：A high performance liquid chromatography method had been established for the determination of malachite green and leucomalachite green in aquatic water.Malachite green and leucomalachite green were extracted with dichloromethane,then concentrated,and dissolved with acetonitrile.Samples were chromatographed using an acetonitrile-ammonium acetate buffer mobile phase on a C18 column in line with a post column reactor filled with lead(IV) oxide and celite.The linear range was from 0.01 μg·mL-1 to 1 μg·mL1 with r2 of 0.999 9.The average recoveries of MG were 83.00%,86.40%,93.50%,and LMG were 74.00%,89.00%,91.80% when samples were spiked with MG and LMG of 0.1,0.5,1.0 μg·mL-1 respectively.The detection limits of MG and LMG were 0.003 μg·mL-1 and 0.004 μg·mL-1 respectively.The RSDs were 4.278%-6.250% and 2.179%-6.757% respectively.The method is sensitive,accurate and suitable for the determination of trace malachite green and leucomalachite green in aquatic water.

关 键 词：孔雀石绿 无色孔雀石绿  高效液相色谱法 养殖水体

分 类 号：S965]