文献类型：期刊文章

作　　者：杨至德[1] 王雁[2] 刘雪梅[1] 缪崑[2] 汤巧香[1]

机构地区：[1]天津城市建设学院规划与建筑系,天津300384 [2]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091

出　　处：《林业科学研究》

基　　金：国家林业局948项目(2004-4-12)的部分研究内容

年　　份：2007

卷　　号：20

期　　号：2

起止页码：292-295

语　　种：中文

收录情况：AJ、BDHX、BDHX2004、BIOSISPREVIEWS、CAB、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、GEOBASE、IC、JST、PROQUEST、RCCSE、SCOPUS、WOS、ZGKJHX、ZR、核心刊

摘　　要：The paper explored the possibility of hairy root occurrence of peony induced by Agrobactrium rhizogenes.It showed that R1000 had the highest hairy root inducement with a hairy root rate of 35%,whereas strains A4,MSU-2 and MT232 had little difference in hairy root occurrence.Infect conditions had great influence on hairy root inducement,and the better ones are: acetosyringone concentration,30 mg·L-1;infection time,15 minutes;and culturing conditions of strains,temperature 28 ℃,28 hours shaking culture under dark environment with shaking.Pre-experiment on tissue culture system of peony was conducted,and got better medium composition as: WP+6-BA 0.5 mg·L-1+ IBA 0.1 mg·L-1+GA30.5 mg·L-1for base buds,and MS + 6-BA 2.0 mg·L-1+IBA 0.1 mg·L-1+GA3 0.5 mg·L-1for stem segments.

关 键 词：发根农杆菌 牡丹  侵染 组织培养  毛状根

分 类 号：S685.11]