文献类型：期刊文章

作　　者：杜娟[1] 狄和双[1] 郭亮[2] 李忠浩[1] 蔡亚非[3] 王根林[1]

机构地区：[1]南京农业大学动物科技学院,南京210095 [2]安徽科技学院动物科学系,凤阳233100 [3]安徽师范大学生命科学学院,芜湖241000

出　　处：《动物学报》

基　　金：*国家科技部十五重大奶业专项( No.2002BA518A12);江苏省"十五"科技攻关项目( No.BG2003303);2005年度江苏省"研究生"创新计划项目;安徽省教育厅自然科学研究项目(No.2006KJ201B)~~

年　　份：2006

卷　　号：52

期　　号：5

起止页码：959-965

语　　种：中文

收录情况：BDHX、BDHX2004、BIOSISPREVIEWS、CAB、CAS、CSA-PROQEUST、CSCD、CSCD2011_2012、DOAJ、EBSCO、IC、JST、PROQUEST、PUBMED、SCIE、SCOPUS、WOS、ZGKJHX、ZR、核心刊

摘　　要：To study the biological and morphological characters of bovine mammary epithelial cells in response to heat stress, the cells were exposed to temperatures from 37 ℃to 41℃ for 1 hour, and the cell activities and morphological characters were measured by MTT, trypan blue dying, transmission microscopy, and flow cytometry. Results were shown as follows: (1) Cell proliferation was inhibited. The cell number in the treatment groups was significantly lower than that in the control(P<0.05). During the recovery period, 6-18 hours at 37 ℃ after treatment,the cell numbers were lower and mitotic phases were shorter in heat-treated groups (treated for more than 5 days)than those in the control group(P<0.05). (2)In response to heat stress, the mammary cell nuclei shrank and splintered,the organelles deformed and vacuolated, and the damaged cells developed into apoptosis bodies.(3)By flow cytometric analysis,the numbers of mammary cells arrested at G2 phase after heat shock was significantly higher than in controls(P<0.01). It is concluded that heat stress inhibites mammary epithelial cell proliferation and induces cell apoptosis.

关 键 词：乳腺上皮细胞 细胞增殖 凋亡

分 类 号：S858.23] Q255[动物医学类]