文献类型：期刊文章

作　　者：何雅青[1] 杨洪[1] 李琳琳[2] 谭洁[1] 毛丽莎[1] 阳帆[1] 刘建军[1] 吕星[1] 周丽[1]

机构地区：[1]深圳市疾病预防控制中心,深圳518020 [2]中国疾病预防控制中心病毒基因工程国家重点实验室,北京100176

出　　处：《病毒学报》

年　　份：2006

卷　　号：22

期　　号：5

起止页码：397-399

语　　种：中文

收录情况：BDHX、BDHX2004、BIOSISPREVIEWS、CAB、CAS、CSCD、CSCD2011_2012、IC、JST、PUBMED、RCCSE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：20 enteroviruses type 71(EV71)were isolated from 410 patients with hand-foot-and-mouth disease in Shenzhen from 2001 to 2004.All of the 20 enteroviruses had been tested by RT-PCR using EV71 VP1 gene specific primers,the positive isolation rate was 4.88%.The amplicons of 341bp segment were cloned into pGEM-T and sequenced.A phylogenetic tree was constructed for comparison of these sequences with those of EV71 genotypes A,B and C using TreeView and PHYLIP software(3.6b).The sequence analysis showed that 20 EV71 strains of Shenzhen shared 81.2%-85.3% nucleotide identity with genotypes A and B.The nucleotide identities among 20 strains were 92.0%-100%.They shared 92.0%-97.9% nucleotide identity with EV71 genotype C4,and can be denominated as EV71 subgenotype C4.This research shows that part of the VP1 gene(341bp) can be used for phylogenetic analysis and pathogen surveillance.

关 键 词：肠道病毒71型 同源性 系统进化分析

分 类 号：R373.2]