文献类型：期刊文章

作　　者：林雪[1] 王仲孚[1] 黄琳娟[1] 白泉[2] 贾敬芬[1]

机构地区：[1]西北大学生命科学学院陕西省生物技术重点实验室西部资源生物与现代生物技术省部共建教育部重点实验室 [2]西北大学分离科学研究所,西安710069

出　　处：《高等学校化学学报》

基　　金：国家重点基础研究发展计划项目(批准号:2004CB520802);陕西省重点实验室重点科研发展计划项目(批准号:05JS49)资助

年　　份：2006

卷　　号：27

期　　号：8

起止页码：1456-1458

语　　种：中文

收录情况：AJ、BDHX、BDHX2004、CAS、CSCD、CSCD2011_2012、EI、IC、JST、RCCSE、RSC、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：An improved carbohydrate derivatization method employing 1-phenyl-3-methyl-5-pyrazolone(PMP) was developed in this study.Liquid ammonia was used instead of sodium hydroxide aqueous solution to provide the base medium for the derivatization reaction.Derivatization reaction and HPLC separation conditions were optimized,including reaction time,reaction temperature and PMP consumption.The derivatives of five reducing monosaccharides were well separated by HPLC. Because we employed ammonia instead of sodium hydro-xide,the excessive volatile ammonia can be removed by vacumm drying,these derivatives could be directly analyzed by MALDI-TOF-MS without desalting procedure,possessing potential application for monosaccharide and oligosaccharide separation and subsequent MS analysis.This method not only increased the MS detection sensitivity,but also decreased sample wastes during purification. We successfully applied this method to mo-nosaccharide composition analysis of polysaccharides SPPA-1,SPPB-1 and SPPC-1 purified from Spirulina(Platensis).

关 键 词：HPLC 1-苯基-3-甲基-5-吡唑啉(PMP)  衍生化 糖  

分 类 号：O658]